Journal article
Adenovirus vector produced Zika virus-like particles induce a long-lived neutralising antibody response in mice
J Carrera, TE Aktepe, L Earnest, D Christiansen, AK Wheatley, HX Tan, AW Chung, S Collett, K McPherson, J Torresi, JM Mackenzie, CP Simmons
Vaccine | Published : 2023
Abstract
Countermeasures against Zika virus (ZIKV) epidemics are urgently needed. In this study we generated a ZIKV virus-like particle (VLP) based vaccine candidate and assessed the immunogenicity of these particles in mice. The ZIKV-VLPs were morphologically similar to ZIKV by electron microscopy and were recognized by anti-Flavivirus neutralising antibodies. We observed that a single dose of unadjuvanted ZIKV-VLPs, or inactivated ZIKV, generated an immune response that lasted over 6 months, but did not neutralize ZIKV infection of cells in vitro. However, when we co-administered the ZIKV VLPs with either Aluminium hydroxide (Alhydrogel®; Alum), AddaVax or Pam2Cys we observed that Alum was the most..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
Acknowledgements We thank VIDRL for providing the ZIKV-Asian strain and Jason Roberts for performing the Immuno-EM. The authors acknowledge the facilities, and the scientific and technical assistance, of the University of Melbourne's Biological Optical Microscopy Platform (BOMP) and Ian Holmes Imaging Centre (IHIC) . This project was supported by grant funding to J.M.M. (NHMRC Project grant No. 1081786) .